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CD3 epsilon antibody [C3e/1308]

Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481
Anti-CD3 epsilon antibody [C3e/1308] used in Flow cytometry (FACS). GTX34481
Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481

Cat. No. GTX34481

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C3e/1308

Isotype

 IgG2b

Application

 WB, FACS

Reactivity

 Human
Package
100 μg ($529)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml for 60 minutes at RT
FACS 1-2μg/10⁶ cells
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CD3e fragment (aa 23-119)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3E , CD3e molecule , IMD18 , T3E , TCRE , CD3 epsilon

Cellular Localization

Membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481

GTX34481 WB Image

WB analysis of recombinant CD3e protein and Jurkat cell lysates using GTX34481 CD3 epsilon antibody [C3e/1308].

Anti-CD3 epsilon antibody [C3e/1308] used in Flow cytometry (FACS). GTX34481

GTX34481 FACS Image

FACS analysis of Jurkat cells using GTX34481 CD3 epsilon antibody [C3e/1308].
Blue : Primary antibody
Red : Isotype control

Anti-CD3 epsilon antibody [C3e/1308] used in Western Blot (WB). GTX34481

GTX34481 WB Image

WB analysis of Jurkat cell lysate using GTX34481 CD3 epsilon antibody [C3e/1308].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529