CD31 antibody [HEC7]

Name: CD31 antibody [HEC7]
Brand: GeneTex
SKU: GTX15842
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CD31 antibody [HEC7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15842

Anti-CD31 antibody [HEC7] used in Flow cytometry (FACS). GTX15842

Cat. No. GTX15842

Host	Mouse
Clonality	Monoclonal
Clone Name	HEC7
Isotype	IgG2a
Application	WB, ICC/IF, FACS, IP, ELISA, IHC, Neutralizing/Inhibition
Reactivity	Human, Rat, Bovine
Package	100 μg ($399)

See all CD31 products

Datasheet
Document

View product citations for antibody GTX15842 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:50-1:500
FACS	3-5 μg/10⁶ cells
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent
Neutralizing/Inhibition	Assay dependent

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PECAM-1

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet and endothelial cell adhesion molecule 1 , CD31 , CD31/EndoCAM , GPIIA'' , PECA1 , PECAM-1 , endoCAM

Cellular Localization

Cell membrane,Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Gene ID: 5175 PECAM1
UniProt: P16284 PECAM1

Research Area

DATA IMAGES

GTX15842 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

GTX15842 FACS Image

FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15842 FACS Image

FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

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REVIEW