CD32a antibody [20D1]

Cat. No. GTX84508

Host	Mouse
Clonality	Monoclonal
Clone Name	20D1
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Monkey
Package	100 μl ($519)

See all CD32a products

Datasheet
Document

View product citations for antibody GTX84508 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
IHC-P	1:50
IP	2ug/500ul

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgG receptor IIa , CD32 , CD32A , CDw32 , FCG2 , FCGR2 , FCGR2A1 , FcGR , IGFR2

Cellular Localization

Cell membrane

Background

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.

Database

Gene ID: 2212 FCGR2A
UniProt: P12318 FCGR2A

Research Area

DATA IMAGES

GTX84508 WB Image

WB analysis of various cell lines using GTX84508 CD32a antibody [20D1].
Loading : 35 ug per lane

GTX84508 WB Image

WB analysis of HEK293T cells transfected with CD32a plasmid (Right) or empty vector (Left) for 48 hrs using GTX84508 CD32a antibody [20D1].
Loading : 5 ug per lane

GTX84508 IHC-P Image

IHC-P analysis of human colon tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IHC-P Image

IHC-P analysis of human breast tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84508 CD32a antibody [20D1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84508 IP Image

IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84508 CD32a antibody [20D1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

CD32a antibody [20D1]

Cat. No. GTX84508

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84508 WB Image

GTX84508 WB Image

GTX84508 IHC-P Image

GTX84508 IHC-P Image

GTX84508 IHC-P Image

GTX84508 IHC-P Image

GTX84508 IHC-P Image

GTX84508 IHC-P Image

GTX84508 IP Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.