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CD34 antibody [4H11(APG)] (FITC)

Anti-CD34 antibody [4H11(APG)] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18227
Anti-CD34 antibody [4H11(APG)] (FITC) used in Flow cytometry (FACS). GTX18227

Cat. No. GTX18227

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H11(APG)

Isotype

 IgG1

Application

 ICC/IF, FACS

Reactivity

 Human
Package
100 test ($399)
See all CD34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody reacts with Class III epitope on CD34 (Mucosialin). The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Purification

Purified by size-exclusion chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_423133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD34 , CD34 molecule

Cellular Localization

Membrane

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [4H11(APG)] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18227

GTX18227 ICC/IF Image

Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 [4H11[APG]]: PE (GTX18228).

Anti-CD34 antibody [4H11(APG)] (FITC) used in Flow cytometry (FACS). GTX18227

GTX18227 FACS Image

Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 [4H11[APG]] FITC (GTX18227). Total viable cells were used for analysis.

REFERENCE

Application Reference

Lin TK et al. Oxid Med Cell Longev 2019; 2019 : 9537504 Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.
Reactivity : Human
Tsu-Kung Lin et al. Oxidative Medicine and Cellular Longevity 2019; 2019 : Article ID 9537504 Mitochondrial Transfer of Wharton’s Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.
Application : FACS
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399