CD36 antibody [C1C3]

Name: CD36 antibody [C1C3]
Brand: GeneTex
SKU: GTX100642
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-CD36 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100642

Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642

Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642

Cat. No. GTX100642

Reference 17

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CD36 products

View product citations for antibody GTX100642 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

mouse brown adipose

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CD36. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949927

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD36 molecule , BDPLT10 , CHDS7 , FAT , GP3B , GP4 , GPIV , PASIV , SCARB3

Cellular Localization

Membrane

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 948 CD36
UniProt: P16671 CD36

Research Area

DATA IMAGES

GTX100642 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using CD36(GTX100642) antibody at 1:200 dilution.

GTX100642 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

GTX100642 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000.

GTX100642 IHC-P Image

CD36 antibody [C1C3] detects CD36 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
CD36 stained by CD36 antibody [C1C3] (GTX100642) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CD36(GTX100642) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100642 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100642 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.