GeneTex
United States (US)

CD40L / CD154 recombinant protein

Cat No. GTX65047

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein's bioactivity determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. Note: results may vary with PBMC donors.

Calculated MW

16.3 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

10 mM Sodium Phosphate, pH 7.5

Storage

Reconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4ºC for 1 week or –20ºC for future use

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd40 Ligand,Cd154,Cd40L,Higm1,Igm,Imd3,T-Bam,Tnfsf5,Trap,Gp39,Hcd40L,Cd40Lg

Background

CD40 Ligand (CD40L/TRAP) is a type II membrane protein that effectuates the helper function of T cells on resting B cells. GeneTex’s Human soluble CD40 Ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L

Research Area

Package List Price ($)
$ 259