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CD43 antibody [SPN/1766R]

Anti-CD43 antibody [SPN/1766R] used in IHC (Paraffin sections) (IHC-P). GTX34512
Anti-CD43 antibody [SPN/1766R] used in Western Blot (WB). GTX34512
Anti-CD43 antibody [SPN/1766R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34512
Anti-CD43 antibody [SPN/1766R] used in Flow cytometry (FACS). GTX34512

Cat. No. GTX34512

Host

Rabbit

Clonality

Monoclonal

Clone Name

SPN/1766R

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human SPN protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD43 , GALGP , GPL115 , LSN , SPN , sialophorin

Cellular Localization

Membrane

Background

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [SPN/1766R] used in IHC (Paraffin sections) (IHC-P). GTX34512

GTX34512 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34512 CD43 antibody [SPN/1766R].

Anti-CD43 antibody [SPN/1766R] used in Western Blot (WB). GTX34512

GTX34512 WB Image

WB analysis of K562 cell lysate using GTX34512 CD43 antibody [SPN/1766R].

Anti-CD43 antibody [SPN/1766R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34512

GTX34512 ICC/IF Image

ICC/IF analysis of K562 cells using GTX34512 CD43 antibody [SPN/1766R].
Green : Primary antibody
Red : nucleus

Anti-CD43 antibody [SPN/1766R] used in Flow cytometry (FACS). GTX34512

GTX34512 FACS Image

FACS analysis of PFA-fixed K562 cells using GTX34512 CD43 antibody [SPN/1766R].
Blue : Primary antibody
Red : Isotype control

REFERENCE

There are currently no references for CD43 antibody [SPN/1766R] (GTX34512). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD43 antibody [SPN/1766R] (GTX34512). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529