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CD44 antibody [8E2F3]

KO/KD Orthogonal
Anti-CD44 antibody [8E2F3] used in IHC (Paraffin sections) (IHC-P). GTX83114
Anti-CD44 antibody [8E2F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83114
Anti-CD44 antibody [8E2F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83114
Anti-CD44 antibody [8E2F3] used in Western Blot (WB). GTX83114
Anti-CD44 antibody [8E2F3] used in Flow cytometry (FACS). GTX83114
Anti-CD44 antibody [8E2F3] used in IHC (Paraffin sections) (IHC-P). GTX83114

Cat. No. GTX83114

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8E2F3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200-1:1000
IHC-P 1:200-1:1000
FACS 1:200-1:400
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascitic fluid containing 0.03% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 628 and 699 of human CD44 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [8E2F3] used in IHC (Paraffin sections) (IHC-P). GTX83114

GTX83114 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX83114 CD44 antibody [8E2F3].

Anti-CD44 antibody [8E2F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83114

GTX83114 ICC/IF Image

ICC/IF analysis of PANC-1 cells using GTX83114 CD44 antibody [8E2F3].
Green : CD44
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-CD44 antibody [8E2F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83114

GTX83114 ICC/IF Image

ICC/IF analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using GTX83114 CD44 antibody [8E2F3].
Green : CD44
Blue: DRAQ5 fluorescent DNA dye

Anti-CD44 antibody [8E2F3] used in Western Blot (WB). GTX83114

GTX83114 WB Image

WB analysis of HeLa (1) and HUVE-12(2) cell lysate using GTX83114 CD44 antibody [8E2F3].

Anti-CD44 antibody [8E2F3] used in Flow cytometry (FACS). GTX83114

GTX83114 FACS Image

FACS analysis of HeLa cells using GTX83114 CD44 antibody [8E2F3].
Right : CD44
Left : negative control

Anti-CD44 antibody [8E2F3] used in IHC (Paraffin sections) (IHC-P). GTX83114

GTX83114 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX83114 CD44 antibody [8E2F3].
Green : CD44
Blue: DRAQ5 fluorescent DNA

REFERENCE

Application Reference

Jhih-Ying Chi et al. Clin Transl Med. 2022; 12 (11) : e1099 Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis
Application : ICC/IF
Reactivity : Human
Hsiao YW et al. Clin Transl Med 2022; 12 (1) : e724 Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers.
Application : WB, ICC/IF, IP
Reactivity : Human
Lin CL et al. Sci Rep 2019; 9 (1) : 14613 Topically Applied Cross-Linked Hyaluronan Attenuates the Formation of Spinal Epidural Fibrosis in a Swine Model of Laminectomy.
Application : IHC-P
Reactivity : Pig
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Package List Price ($)
$ 399