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CD52 antibody

KO/KD Orthogonal
Anti-CD52 antibody used in Western Blot (WB). GTX00743
Anti-CD52 antibody used in Western Blot (WB). GTX00743
Anti-CD52 antibody used in IHC (Paraffin sections) (IHC-P). GTX00743
Anti-CD52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00743
Anti-CD52 antibody used in Western Blot (WB). GTX00743

Cat. No. GTX00743

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse
Package
100 μl ($399)
See all CD52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:100
Not tested in other applications.

Calculated MW

8 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to 29-48 amino acids of mouse CD52, C-AASGTNKNSTSTKKTPLKSG , conjugated with KLH

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD52 antigen , AI463198 , B7 , B7-Ag , CAMPATH-1 , CLS1 , MB7

Database

Research Area

DATA IMAGES

Anti-CD52 antibody used in Western Blot (WB). GTX00743

GTX00743 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00743 CD52 antibody.
Samples: testes, male reproductive ducts (30 μg), and sperm proteinmouse tissue proteins (10 μg)
Dilution : 1:1000

Anti-CD52 antibody used in Western Blot (WB). GTX00743

GTX00743 WB Image

KO/KD

WB analysis of wild-type and CD52 deficient mice using GTX00743 CD52 antibody.
Upper : Cauda epididymal lysates (30 μg)
Lower : Sperm lysates (10 μg)
(+/+) : Cd52+/+
(+/-) : Cd52+/-
(-/-) : Cd52-/-
Dilution : 1:1000

Anti-CD52 antibody used in IHC (Paraffin sections) (IHC-P). GTX00743

GTX00743 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00743 CD52 antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

Anti-CD52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00743

GTX00743 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX00743 CD52 antibody.
Dilution : 1:100
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

Anti-CD52 antibody used in Western Blot (WB). GTX00743

GTX00743 WB Image

WB analysis of mouse testis or sperm tissue lysate using GTX00743 CD52 antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399