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CD55 antibody [3D5]

Cat. No. GTX54409

Host

 Rat

Clonality

 Monoclonal

Clone Name

 3D5

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all CD55 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
FACS Assay dependent
ELISA 2μg/ml

Note :

WB
Proteins were separated by SDS-PAGE, transferred to nitrocellulose membranes and blocked with Tris-buffered saline containing 0.05% Tween 20 (TBST),3% non-fat dried milk and 3% BSA.

IHC-P
A mixture of 3D5 and another antibody was used. Colon segments were fixed overnight in 10% Formalin, embedded in paraffin blocks, and cut into 5 μm sections. Sections were stained with H&E.

ELISA
This antibody was used as a detection antibody in a direct ELISA (plates coated with mouse CD55.
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

NRK cells expressing transmembrane-anchored mouse DAF

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD55 molecule, decay accelerating factor for complement , Daf , Daf-GPI , Daf1 , GPI-DAF

Cellular Localization

Cell membrane

Background

This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1. [provided by RefSeq, Nov 2015]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 399