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CD58 antibody [TS2/9]

Anti-CD58 antibody [TS2/9] used in Flow cytometry (FACS). GTX15669
Anti-CD58 antibody [TS2/9] used in Western Blot (WB). GTX15669
Anti-CD58 antibody [TS2/9] used in Flow cytometry (FACS). GTX15669
Anti-CD58 antibody [TS2/9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15669

Cat. No. GTX15669

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TS2/9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all CD58 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:500
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Leukocyte Function Antigen-3

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD58 molecule , LFA-3 , LFA3 , ag3

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-CD58 antibody [TS2/9] used in Flow cytometry (FACS). GTX15669

GTX15669 FACS Image

FACS analysis of PBMC cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4ºC

Anti-CD58 antibody [TS2/9] used in Western Blot (WB). GTX15669

GTX15669 WB Image

WB analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) using GTX15669 CD58 antibody [TS2/9].
Dilution : 1:100

Anti-CD58 antibody [TS2/9] used in Flow cytometry (FACS). GTX15669

GTX15669 FACS Image

FACS analysis of BAF-3 cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-CD58 antibody [TS2/9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15669

GTX15669 ICC/IF Image

ICC/IF analysis of Raji cells using GTX15669 CD58 antibody [TS2/9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations