APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human CD68 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD68 , CD68 molecule , GP110 , LAMP4 , SCARD1
Cellular Localization
Cell membrane,Endosome membrane,Lysosome membrane
Background
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544](/templates/default/js/lightbox/images/loading.gif "Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544")
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GTX34544 ICC/IF Image
ICC/IF analysis of U87MG cells using GTX34544 CD68 antibody [C68/684].
Green : Primary antibody
Red : nucleus
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GTX34544 WB Image
WB analysis of human spleen tissue lysate using GTX34544 CD68 antibody [C68/684].
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GTX34544 FACS Image
FACS analysis of U87MG cells using GTX34544 CD68 antibody [C68/684].
Blue : Primary antibody
Red : Isotype control
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GTX34544 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34544 CD68 antibody [C68/684].
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GTX34544 IHC-P Image
IHC-P analysis of human histiocytoma tissue using GTX34544 CD68 antibody [C68/684].
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REFERENCE
There are currently no references for CD68 antibody [C68/684] (GTX34544). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD68 antibody [C68/684] (GTX34544). Be the first to share your experience with this product.
![Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544](/upload/website/prouct_img/small/GTX34544/GTX34544_20200115_ICC-IF_1912_w_23060801_408.webp "Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544")
![Anti-CD68 antibody [C68/684] used in Western Blot (WB). GTX34544](/upload/website/prouct_img/small/GTX34544/GTX34544_20200115_WB_1178_w_23060801_944.webp "Anti-CD68 antibody [C68/684] used in Western Blot (WB). GTX34544")
![Anti-CD68 antibody [C68/684] used in Flow cytometry (FACS). GTX34544](/upload/website/prouct_img/small/GTX34544/GTX34544_20200115_FACS_1909_w_23060801_775.webp "Anti-CD68 antibody [C68/684] used in Flow cytometry (FACS). GTX34544")
![Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544](/upload/website/prouct_img/small/GTX34544/GTX34544_20200115_IHC-P_1331_w_23060801_797.webp "Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544")
![Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544](/upload/website/prouct_img/small/GTX34544/GTX34544_20200115_IHC-P_618_w_23060801_397.webp "Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544")
![Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544](/upload/website/prouct_img/normal/GTX34544/GTX34544_20200115_ICC-IF_1912_w_23060801_408.webp "Anti-CD68 antibody [C68/684] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34544")
![Anti-CD68 antibody [C68/684] used in Western Blot (WB). GTX34544](/upload/website/prouct_img/normal/GTX34544/GTX34544_20200115_WB_1178_w_23060801_944.webp "Anti-CD68 antibody [C68/684] used in Western Blot (WB). GTX34544")
![Anti-CD68 antibody [C68/684] used in Flow cytometry (FACS). GTX34544](/upload/website/prouct_img/normal/GTX34544/GTX34544_20200115_FACS_1909_w_23060801_775.webp "Anti-CD68 antibody [C68/684] used in Flow cytometry (FACS). GTX34544")
![Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544](/upload/website/prouct_img/normal/GTX34544/GTX34544_20200115_IHC-P_1331_w_23060801_797.webp "Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544")
![Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544](/upload/website/prouct_img/normal/GTX34544/GTX34544_20200115_IHC-P_618_w_23060801_397.webp "Anti-CD68 antibody [C68/684] used in IHC (Paraffin sections) (IHC-P). GTX34544")