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CD74 antibody [LN-2]

Anti-CD74 antibody [LN-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34551
Anti-CD74 antibody [LN-2] used in IHC (Paraffin sections) (IHC-P). GTX34551
Anti-CD74 antibody [LN-2] used in IHC (Paraffin sections) (IHC-P). GTX34551
Anti-CD74 antibody [LN-2] used in Flow cytometry (FACS). GTX34551

Cat. No. GTX34551

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LN-2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, Functional Assay

Reactivity

 Human, Mouse, Baboon
Package
100 μg ($529)
See all CD74 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1-4μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Functional Assay Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SU-DHL-4 lymphoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD74 , CD74 molecule , DHLAG , HLADG , II , IaGAMMA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus,Trans-Golgi network,Endosome,Lysosome

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD74 antibody [LN-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34551

GTX34551 ICC/IF Image

ICC/IF analysis of Raji cells using GTX34551 CD74 antibody [LN-2].
Green : Primary antibody
Red : nucleus

Anti-CD74 antibody [LN-2] used in IHC (Paraffin sections) (IHC-P). GTX34551

GTX34551 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34551 CD74 antibody [LN-2].

Anti-CD74 antibody [LN-2] used in IHC (Paraffin sections) (IHC-P). GTX34551

GTX34551 IHC-P Image

IHC-P analysis of human spleen tissue using GTX34551 CD74 antibody [LN-2].

Anti-CD74 antibody [LN-2] used in Flow cytometry (FACS). GTX34551

GTX34551 FACS Image

FACS analysis of Raji cells using GTX34551 CD74 antibody [LN-2].
Blue : Primary antibody
Red : Isotype control

REFERENCE

Clone Reference

S Ebert et al. Cells 2023; 12 (14):1899 Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation
Application : Functional Assay
Z Berkova et al. Exp Clin Cancer Res 2014; 33 (1):80 CD74 interferes with the expression of fas receptor on the surface of lymphoma cells
Application : WB
M McClelland et al. Am J Pathol 2009; 174 (2):638-46 Expression of CD74, the receptor for macrophage migration inhibitory factor, in non-small cell lung cancer
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529