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CD90 antibody [HL1766]

Recombinant antibody Orthogonal
Anti-CD90 antibody [HL1766] used in IHC (Paraffin sections) (IHC-P). GTX637416
Anti-CD90 antibody [HL1766] used in Western Blot (WB). GTX637416
Anti-CD90 antibody [HL1766] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637416
Anti-CD90 antibody [HL1766] used in Western Blot (WB). GTX637416

Cat. No. GTX637416

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1766

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD90 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD90. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Thy-1 cell surface antigen , CD90 , CDw90

Cellular Localization

Cell membrane

Background

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-CD90 antibody [HL1766] used in IHC (Paraffin sections) (IHC-P). GTX637416

GTX637416 IHC-P Image

CD90 antibody [HL1766] detects CD90 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: CD90 stained by CD90 antibody [HL1766] (GTX637416) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD90 antibody [HL1766] used in Western Blot (WB). GTX637416

GTX637416 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD90 antibody [HL1766] (GTX637416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD90 antibody [HL1766] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637416

GTX637416 ICC/IF Image

CD90 antibody [HL1766] detects CD90 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD90 stained by CD90 antibody [HL1766] (GTX637416) diluted at 1:250.
Red: Tau, an Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD90 antibody [HL1766] used in Western Blot (WB). GTX637416

GTX637416 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD90 antibody [HL1766] (GTX637416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations