APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
Not tested in other applications.
Calculated MW
Product Note
CD97 beta (cleaved Ser531) antibody detects endogenous levels of fragment of activated CD97 beta resulting from cleavage adjacent to Ser531.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human CD97 beta (512-561).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ADGRE5 , CD97 , TM7LN1 , adhesion G proteincoupled receptor E5 , adhesion G protein-coupled receptor E5
Cellular Localization
Cell membrane,Secreted,Extracellular space
Background
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
Database
Research Area
DATA IMAGES
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GTX86961 WB Image
WB analysis of Jurkat cells treated with etoposide 25uM (24h) lysate using GTX86961 CD97 beta (cleaved Ser531) antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for CD97 beta (cleaved Ser531) antibody (GTX86961). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD97 beta (cleaved Ser531) antibody (GTX86961). Be the first to share your experience with this product.
