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CDC25C antibody [1F12]

Anti-CDC25C antibody [1F12] used in Western Blot (WB). GTX82998
Anti-CDC25C antibody [1F12] used in IHC (Paraffin sections) (IHC-P). GTX82998

Cat. No. GTX82998

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F12

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CDC25C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDC25C(AA : 1-200) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 25C , CDC25 , PPP1R60

Cellular Localization

Nucleus

Background

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CDC25C antibody [1F12] used in Western Blot (WB). GTX82998

GTX82998 WB Image

WB analysis of HeLa (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264 using GTX82998 CDC25C antibody [1F12].

Anti-CDC25C antibody [1F12] used in IHC (Paraffin sections) (IHC-P). GTX82998

GTX82998 IHC-P Image

IHC-P analysis of human Testis tissue using GTX82998 CDC25C antibody [1F12].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399