CDC34 antibody [14B5]

Cat. No. GTX70125

Host	Mouse
Clonality	Monoclonal
Clone Name	14B5
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399)

See all CDC34 products

Datasheet

View product citations for antibody GTX70125 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293 cells and recombinant fusion protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human Cdc34 expressed in E. coli.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

RRID

AB_377582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell division cycle 34 , E2-CDC34 , UBC3 , UBCH3 , UBE2R1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]

Database

Gene ID: 997 CDC34
UniProt: P49427 CDC34

Research Area

DATA IMAGES

GTX70125 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HCT116
10% SDS PAGE
GTX70125 diluted at 1:1000

GTX70125 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using CDC34(GTX70125) antibody at 1:200 dilution.

GTX70125 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDC34(GTX70125) antibody at 1:100 dilution.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

Qty Custom Conjugations