APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150 to 250 of human.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
F-box and WD repeat domain containing 7 , AGO , CDC4 , FBW6 , FBW7 , FBX30 , FBXO30 , FBXW6 , SEL-10 , SEL10 , hAgo , hCdc4
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX04244 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX04244 CDC4 antibody. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2.
Dilution : 1:100
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GTX04244 WB Image
WB analysis of SH-SY5Y cell lysates using GTX04244 CDC4 antibody.
Loading : 25 μg
Dilution : 1:500
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GTX04244 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX04244 CDC4 antibody. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2.
Dilution : 1:100
|
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GTX04244 WB Image
WB analysis of various tissue lysates using GTX04244 CDC4 antibody.
Loading : 25 μg
Dilution : 1:500
|
REFERENCE
There are currently no references for CDC4 antibody (GTX04244). Be the first to share your publications with this product.
REVIEW
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