GeneTex
United States (US)

CDKN2A / p14ARF antibody

p14ARF-antibody_WB_GTX14930-1_18121411_250.jpg

Cat No. GTX14930

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

WB: Use at a dilution of 1/500. Predicted molecular weight: 17 kDa. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: GRCVGPSLQLRGQEWRCS (Human) (N terminal).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Multiple tumo, p14ARF, Cyclin dependent kinase inhibitor 2A, TP16, Cyclin dependent kinase 4 inhibitor A, MTS1, Cell cycle negative regulator beta, CDKN2A, CMM2, MLM, Cyclin dependent kinase inhibitor2A, Cyclin dependent kinase inhibitor P12, Cyclin dependent kinase4 inhibitor A, Melanoma p16 inhibits CDK4, P19, P14, ARF, CDK4I, CDKN2, INK4, INK4A, MTS-1, P16, P16-INK4A, P16INK4, P16INK4A, P19ARF, MTS 1, P16 INK4A

Background

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

Research Area

DATA IMAGES
p14ARF-antibody_WB_GTX14930-1_18121411_250.jpg

GTX14930 WB Image

Western blot of ARF1 antibody. Antibody dilution 1:500 in diluObuffer. MW of ARF1 is 21kDa.