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CDT2 antibody

KO/KD
Anti-CDT2 antibody used in Western Blot (WB). GTX00893
Anti-CDT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00893
Anti-CDT2 antibody used in Western Blot (WB). GTX00893
Anti-CDT2 antibody used in Western Blot (WB). GTX00893

Cat. No. GTX00893

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μl ($399)
See all CDT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

E. coli expressed and purified 6xHis-tagged C-terminal 150 amino acids of human Cdt2

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDT2 , DCAF2 , DTL , L2DTL , RAMP , denticleless E3 ubiquitin protein ligase homolog

Cellular Localization

Nucleus,Nucleus membrane,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Chromosome

Database

Research Area

DATA IMAGES

Anti-CDT2 antibody used in Western Blot (WB). GTX00893

GTX00893 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control or Cdt2 siRNA using GTX00893 CDT2 antibody. Cdt2 is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).

Anti-CDT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00893

GTX00893 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00893 CDT2 antibody. Cdt2 protein is localized in nuclei at all cell stages.
Fixation : 4% PFA, 10 min
Permeabilization : 0.25% (v/v) Triton X-100 in PBS

Anti-CDT2 antibody used in Western Blot (WB). GTX00893

GTX00893 WB Image

WB analysis of HeLa whole cell lysate using GTX00893 CDT2 antibody.
Dilution : 1:2000
Loading : 20 μg

Anti-CDT2 antibody used in Western Blot (WB). GTX00893

GTX00893 WB Image

WB analysis of various samples using GTX00893 CDT2 antibody. Doublet bands reflect post-translational phospholylation.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Lane 4 : CHO whole cell lysate
Dilution : 1:200
Loading : 40 μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399