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CENPE antibody [1H12]

Cat. No. GTX25093

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H12

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
50 μl ($399)
See all CENPE products

APPLICATION

Application Note

Recommended Starting Dilutions:
For ICC/IF: Use at 0.5-1μg/ml
For IP: Use approximately 3μg/ml of antibody for 300μg of cell lysate
Optimal dilutions should be determined experimentally by the researcher.

Product Note

Human CENP-E. Not tested on reactivity with rodents or other vertebrates

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length CENP-E protein (human)

Purification

Affinity purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Centromere Protein E , Cenp-E , Kif10 , Mcph13 , Ppp1R61 , Cenpe

Background

CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation.

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399