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CENPF antibody

Anti-CENPF antibody used in Western Blot (WB). GTX30232
Anti-CENPF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30232
Anti-CENPF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30232

Cat. No. GTX30232

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Bovine
Package
50 μl ($419)
See all CENPF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1500 - 1:2500
ICC/IF 1:200 - 1:1000
FACS 1:50 - 1:200
IP 1:50
Not tested in other applications.

Calculated MW

358 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_367488

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein F , CENF , CILD31 , PRO1779 , STROMS , hcp-1

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CENPF antibody used in Western Blot (WB). GTX30232

GTX30232 WB Image

WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody.
Loading : 25μg

Anti-CENPF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30232

GTX30232 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30232 CENPF antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:5

Anti-CENPF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30232

GTX30232 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30232 CENPF antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

Application Reference

Chung HJ et al. J Biol Chem 2016; (Epub) Phosphorylation of Astrin regulates its Kinetochore Function.
Application : ICC/IF
Reactivity : Human
Rodrigue A et al. J Cell Sci 2013; 126 (Pt 1) : 348-59 The RAD51 paralogs ensure cellular protection against mitotic defects and aneuploidy.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419