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CHD4 antibody

Anti-CHD4 antibody used in Western Blot (WB). GTX101662
Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX101662
Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101662
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662

Cat. No. GTX101662

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CHD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

218 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165489

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromodomain helicase DNA binding protein 4 , CHD-4 , Mi-2b , Mi2-BETA , SIHIWES

Cellular Localization

Nucleus

Background

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CHD4 antibody used in Western Blot (WB). GTX101662

GTX101662 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody (GTX101662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX101662

GTX101662 IP Image

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX101662).
Western blot analysis was performed using CHD4 antibody (GTX101662) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101662

GTX101662 ICC/IF Image

CHD4 antibody detects CHD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662

GTX101662 IHC-P Image

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662

GTX101662 IHC-P Image

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662

GTX101662 IHC-P Image

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101662

GTX101662 IHC-P Image

CHD4 antibody detects CHD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
CHD4 stained by CHD4 antibody (GTX101662) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169