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CHD4 antibody

KO/KD Orthogonal Overexpression
Anti-CHD4 antibody used in Western Blot (WB). GTX124186
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186
Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124186
Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX124186
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186
Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX124186
Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186
Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124186
Anti-CHD4 antibody used in Western Blot (WB). GTX124186

Cat. No. GTX124186

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CHD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

218 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromodomain helicase DNA binding protein 4 , CHD-4 , Mi-2b , Mi2-BETA , SIHIWES

Cellular Localization

Nucleus

Background

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CHD4 antibody used in Western Blot (WB). GTX124186

GTX124186 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX124186 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186

GTX124186 IHC-P Image

CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
CHD4 antibody (GTX124186) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124186

GTX124186 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4(GTX124186) antibody at 1:500 dilution.

Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX124186

GTX124186 IP Image

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX124186) or CHD4 antibody (GTX101662).
Western blot analysis was performed using CHD4 antibody (GTX124186).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186

GTX124186 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using CHD4(GTX124186) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHD4 antibody used in Immunoprecipitation (IP). GTX124186

GTX124186 IP Image

Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody (GTX124186).
Western blot analysis was performed using CHD4 antibody (GTX124186) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX124186

GTX124186 IHC-P Image

CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
CHD4 antibody (GTX124186) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CHD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124186

GTX124186 ICC/IF Image

Orthogonal

The data was published in the journal Cell Discov in 2015.PMID: 27462424

Anti-CHD4 antibody used in Western Blot (WB). GTX124186

GTX124186 WB Image

KO/KD Orthogonal Overexpression

The data was published in the journal Int J Mol Sci in 2019. PMID: 31438571

REFERENCE

Application Reference

Chang CL et al. Int J Biol Sci 2021; 17 (7) : 1660-1670 CHD4 as an important mediator in regulating the malignant behaviors of colorectal cancer.
Application : WB
Reactivity : Human
Ou-Yang F et al. Life Sci 2019; 238 : 116963 Identification of CHD4-帣1 integrin axis as a prognostic marker in triple-negative breast cancer using next-generation sequencing and bioinformatics.
Application : WB
Reactivity : Human
Wang HC et al. Int J Mol Sci 2019; 20 (17) Over-Expression of CHD4 Is an Independent Biomarker of Poor Prognosis in Patients with Rectal Cancers Receiving Concurrent Chemoradiotherapy.
Application : WB, IHC-P
Reactivity : Human
Luo CW et al. Exp Cell Res 2018; (Epub) CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
Application : WB, IHC-P
Reactivity : Human
Hou MF et al. Exp Cell Res 2017; 359 (2) : 458-465 The NuRD complex-mediated p21 suppression facilitates chemoresistance in BRCA-proficient breast cancer.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169