GeneTex

  • Country / Location Selection

United States (US)

CIRBP antibody [N1C3]

Anti-CIRBP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120184
Anti-CIRBP antibody [N1C3] used in Western Blot (WB). GTX120184
Anti-CIRBP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120184

Cat. No. GTX120184

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CIRBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CIRBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721018

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cold inducible RNA binding protein , CIRP

Cellular Localization

Nucleus , nucleoplasm

Database

Research Area

DATA IMAGES

Anti-CIRBP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120184

GTX120184 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CIRBP(GTX120184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CIRBP antibody [N1C3] used in Western Blot (WB). GTX120184

GTX120184 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX120184 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CIRBP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120184

GTX120184 ICC/IF Image

CIRBP antibody [N1C3] detects CIRBP protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CIRBP protein stained by CIRBP antibody [N1C3] (GTX120184) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for CIRBP antibody [N1C3] (GTX120184). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169