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CKAP4 antibody [N3C3]

Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087
Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087
Anti-CKAP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120087
Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087
Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087
Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087

Cat. No. GTX120087

4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CKAP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CKAP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720164

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytoskeleton associated protein 4 , CLIMP-63 , ERGIC-63 , p63

Cellular Localization

Endoplasmic reticulum-Golgi intermediate compartment membrane

Database

Research Area

DATA IMAGES

Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087

GTX120087 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087

GTX120087 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CKAP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120087

GTX120087 ICC/IF Image

CKAP4 antibody [N3C3] detects CKAP4 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087

GTX120087 IHC-P Image

CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087

GTX120087 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CKAP4(GTX120087) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CKAP4 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX120087

GTX120087 IHC-P Image

CKAP4 antibody [N3C3] detects CKAP4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for CKAP4 antibody [N3C3] (GTX120087). Be the first to share your publications with this product.
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REVIEW

CKAP4 antibody [N3C3] Cat. No. GTX120087

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-CKAP4 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120087
Date : Anonymous submitted on 23-Feb-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U2OS cells
Fixation : 3.7 % formaldehyde in PBS
Permeabilization /Antigen retrieval : 0.5 % Triton X-100 in PBS
Primary Antibody : 1:400, 25°C, 1Hr
Anti-CKAP4 antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX120087
Date : Anonymous submitted on 23-Feb-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Sample : HeLa cells
Blocking : 3 % skim milk in PBS, 25°C, 0.5Hr
Lane Description : HeLa total cell lysate (2 x 10^6 cells/IP)
lane 1: input
lane 2: rabbit anti-IgG (control, 5 ug)
lane 3 GTX120087 (2.5 ug)
WB: GTX120087 (X1000)
Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : aortic smooth muscle cells
Amount used : 4.5μg
Blocking : 3% milk in PBST, 23°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Anti-CKAP4 antibody [N3C3] used in Western Blot (WB). GTX120087
Date : Anonymous submitted on 21-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169