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CLCN5 antibody

Overexpression
Anti-CLCN5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53963
Anti-CLCN5 antibody used in Western Blot (WB). GTX53963

Cat. No. GTX53963

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CLCN5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 637-816 of human CLCN5 (NP_001121370.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chloride voltage-gated channel 5 , CLC5 , CLCK2 , ClC-5 , DENTS , NPHL1 , NPHL2 , XLRH , XRN , hCIC-K2

Cellular Localization

Golgi apparatus membrane,Endosome membrane,Cell membrane

Background

This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-CLCN5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53963

GTX53963 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX53963 CLCN5 antibody.
Blue : DAPI

Anti-CLCN5 antibody used in Western Blot (WB). GTX53963

GTX53963 WB Image

WB analysis of various sample lysates using GTX53963 CLCN5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Yadav MK et al. Mol Ther Methods Clin Dev 2022; 27 : 149-166 Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice.
Application : WB
Reactivity : Human, Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399