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CLEC4E antibody [AT16E3]

Anti-CLEC4E antibody [AT16E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53742
Anti-CLEC4E antibody [AT16E3] used in Western Blot (WB). GTX53742
Anti-CLEC4E antibody [AT16E3] used in IHC (Paraffin sections) (IHC-P). GTX53742

Cat. No. GTX53742

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT16E3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CLEC4E products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CLEC4E (41-219aa) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-type lectin domain family 4 member E , CLECSF9 , MINCLE

Cellular Localization

Membrane

Background

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CLEC4E antibody [AT16E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53742

GTX53742 ICC/IF Image

ICC/IF analysis of HeLa cells using MINCLE antibody antibody at a dilution of 1:500 (Red). Nuclei were stained by Hoechst 33342 (blue).

Anti-CLEC4E antibody [AT16E3] used in Western Blot (WB). GTX53742

GTX53742 WB Image

WB analysis of HeLa lysate (35ug) using MINCLE antibody at a dilution of 1:1,000.

Anti-CLEC4E antibody [AT16E3] used in IHC (Paraffin sections) (IHC-P). GTX53742

GTX53742 IHC-P Image

IHC-P analysis of colorectal cancer tissue using MINCLE antibody at a dilution of 1:50.

REFERENCE

Application Reference

Hitoshi Nakayama et al. Tuberculosis 2023; Phylactic role of anti-lipoarabinomannan IgM directed against mannan core during mycobacterial infection in macrophages
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399