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CMPK1 antibody [N1C3]

Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566
Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566
Anti-CMPK1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105566

Cat. No. GTX105566

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CMPK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Raji , Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CMPK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytidine/uridine monophosphate kinase 1 , CK , CMK , CMPK , UMK , UMP-CMPK , UMPK

Cellular Localization

Nucleus , Cytoplasm

Background

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566

GTX105566 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX105566 diluted at 1:500

Anti-CMPK1 antibody [N1C3] used in Western Blot (WB). GTX105566

GTX105566 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105566 diluted at 1:1000

Anti-CMPK1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105566

GTX105566 ICC/IF Image

CMPK1 antibody [N1C3] detects CMPK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CMPK1 protein stained by CMPK1 antibody [N1C3] (GTX105566) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169