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CNPase antibody

KO/KD Orthogonal
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954
Anti-CNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954
Anti-CNPase antibody used in Western Blot (WB). GTX103954

Cat. No. GTX103954

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CNPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain , HeLa

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CNPase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036608

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2',3'-cyclic nucleotide 3' phosphodiesterase , CNP1

Cellular Localization

Membrane , Melanosome

Database

Research Area

DATA IMAGES

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

KO/KD

Wild-type (WT) and CNPase knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNPase antibody used in IHC (Frozen sections) (IHC-Fr). GTX103954

GTX103954 IHC-Fr Image

CNPase antibody detects CNPase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: CNPase protein stained by CNPase antibody (GTX103954) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNP antibody detects CNP protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
CNP antibody (GTX103954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNPase antibody detects CNPase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CNPase stained by CNPase antibody (GTX103954) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNP antibody (GTX103954) diluted at 1:5000.

Anti-CNPase antibody used in IHC (Paraffin sections) (IHC-P). GTX103954

GTX103954 IHC-P Image

CNPase antibody detects CNPase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CNPase stained by CNPase antibody (GTX103954) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CNPase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103954

GTX103954 ICC/IF Image

CNPase antibody detects CNPase protein at cell membrane by immunofluorescent analysis.
Sample: MOLT-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNPase stained by CNPase antibody (GTX103954) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CNPase antibody (GTX103954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CNPase antibody used in Western Blot (WB). GTX103954

GTX103954 WB Image

KO/KD Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

REFERENCE

Application Reference

Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169