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COL11A2 antibody [GT212]

Anti-COL11A2 antibody [GT212] used in Western Blot (WB). GTX629481
Anti-COL11A2 antibody [GT212] used in Western Blot (WB). GTX629481

Cat. No. GTX629481

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT212

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COL11A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

172 kDa. ( Note )

Positive Control

human COLC11A2-transfected 293T cells (partial fragment) , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human COL11A2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888119

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type XI alpha 2 chain , DFNA13 , DFNB53 , FBCG2 , HKE5 , OSMEDA , OSMEDB , PARP , STL3

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Three transcript variants encoding different isoforms have been identified for this gene. A pseudogene is located nearby on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COL11A2 antibody [GT212] used in Western Blot (WB). GTX629481

GTX629481 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [GT212] (GTX629481) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COL11A2 antibody [GT212] used in Western Blot (WB). GTX629481

GTX629481 WB Image

COL11A2 antibody [GT212] detects COL11A2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human COLC11A2-transfected 293T cells (partial fragment)
12 % SDS-PAGE
COL11A2 antibody [GT212] (GTX629481) dilution: 1:10000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations