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COL4A3BP antibody

Anti-COL4A3BP antibody used in IHC (Paraffin sections) (IHC-P). GTX122753
Anti-COL4A3BP antibody used in Western Blot (WB). GTX122753

Cat. No. GTX122753

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all COL4A3BP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COL4A3BP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174320

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type IV alpha 3 binding protein , CERT , CERTL , GPBP , MRD34 , STARD11

Cellular Localization

Cytoplasm , Golgi apparatus , Endoplasmic reticulum

Background

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COL4A3BP antibody used in IHC (Paraffin sections) (IHC-P). GTX122753

GTX122753 IHC-P Image

COL4A3BP antibody detects COL4A3BP protein at cytosol on H441 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H441 xenograft.
COL4A3BP antibody (GTX122753) dilution: 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A3BP antibody used in Western Blot (WB). GTX122753

GTX122753 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX122753 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169