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COL4A6 antibody

Anti-COL4A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56141
Anti-COL4A6 antibody used in Western Blot (WB). GTX56141

Cat. No. GTX56141

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all COL4A6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of COL4A6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type IV alpha 6 chain , CXDELq22.3 , DELXq22.3 , DFNX6

Background

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-COL4A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56141

GTX56141 ICC/IF Image

ICC/IF analysis of formalin-fixed K562 cells using GTX56141 COL4A6 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-COL4A6 antibody used in Western Blot (WB). GTX56141

GTX56141 WB Image

WB analysis of K562 (A), rat heart (B) whole cell lysates using GTX56141 COL4A6 antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399