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COL6A1 antibody [C1C2], Internal

KO/KD Orthogonal
Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963
Anti-COL6A1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109963
Anti-COL6A1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109963
Anti-COL6A1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109963
Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963
Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963

Cat. No. GTX109963

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COL6A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

U87-MG , 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COL6A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036623

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type VI alpha 1 chain , BTHLM1 , OPLL , UCHMD1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963

GTX109963 WB Image

KO/KD

Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL6A1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109963

GTX109963 IHC-P Image

COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL6A1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109963

GTX109963 IHC-P Image

COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COL6A1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109963

GTX109963 ICC/IF Image

COL6A1 antibody [C1C2], Internal detects COL6A1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL6A1 stained by COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963

GTX109963 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL6A1 antibody [C1C2], Internal used in Western Blot (WB). GTX109963

GTX109963 WB Image

The data was published in the 2022 in Clin Exp Dent Res. PMID: 35106969

REFERENCE

Application Reference

Choi Y et al. Biomolecules 2022; 12 (12) Characterization of Transcriptomic and Proteomic Changes in the Skin after Chronic Fluocinolone Acetonide Treatment.
Application : WB
Reactivity : Mouse
Ruiter FAA et al. Adv Sci (Weinh) 2022; 9 (20) : e2200543 Soft, Dynamic Hydrogel Confinement Improves Kidney Organoid Lumen Morphology and Reduces Epithelial-Mesenchymal Transition in Culture.
Application : IHC-Fr
Reactivity : Human
Passanha FR et al. Stem Cells 2022; 40 (7) : 669-677 Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGFβ1 Pathway.
Application : WB, ICC/IF
Reactivity : Human
Hosiriluck N et al. Clin Exp Dent Res 2022; (Epub) The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169