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COPB antibody

Anti-COPB antibody used in Western Blot (WB). GTX22915
Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22915

Cat. No. GTX22915

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Rat
Package
100 μg ($399)
See all COPB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues K(494) V L A A Q E T H E G V T E D G I E(511) of rat beta-COP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coatomer protein complex, subunit beta 1

Cellular Localization

Cytoplasm,Golgi apparatus membrane,Cell membrane

Background

transmembrane protein associated with the Golgi; may be a component of non-clathrin coated vesicles and cisternae of the Golgi complex [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-COPB antibody used in Western Blot (WB). GTX22915

GTX22915 WB Image

WB analysis of PC-12 cell lysates using GTX22915 COPB antibody.

Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22915

GTX22915 ICC/IF Image

ICC/IF analysis of L6 cells using GTX22915 COPB antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399