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COTL1 antibody [5C8]

Anti-COTL1 antibody [5C8] used in ELISA (ELISA). GTX60551
Anti-COTL1 antibody [5C8] used in Western Blot (WB). GTX60551
Anti-COTL1 antibody [5C8] used in Western Blot (WB). GTX60551
Anti-COTL1 antibody [5C8] used in IHC (Paraffin sections) (IHC-P). GTX60551
Anti-COTL1 antibody [5C8] used in IHC (Paraffin sections) (IHC-P). GTX60551

Cat. No. GTX60551

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C8

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all COTL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human COTL1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coactosin like F-actin binding protein 1 , CLP

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-COTL1 antibody [5C8] used in ELISA (ELISA). GTX60551

GTX60551 ELISA Image

ELISA analysis of antigen using GTX60551 COTL1 antibody [5C8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-COTL1 antibody [5C8] used in Western Blot (WB). GTX60551

GTX60551 WB Image

WB analysis of human COTL1 (AA: 1-142) recombinant protein using GTX60551 COTL1 antibody [5C8].

Anti-COTL1 antibody [5C8] used in Western Blot (WB). GTX60551

GTX60551 WB Image

WB analysis of HEK293 (1) and COTL1 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate using GTX60551 COTL1 antibody [5C8].

Anti-COTL1 antibody [5C8] used in IHC (Paraffin sections) (IHC-P). GTX60551

GTX60551 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60551 COTL1 antibody [5C8].

Anti-COTL1 antibody [5C8] used in IHC (Paraffin sections) (IHC-P). GTX60551

GTX60551 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60551 COTL1 antibody [5C8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409