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COX4 antibody [GT854]

KO/KD Orthogonal
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

Cat. No. GTX628901

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT854

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

mouse heart , rat muscle , 293T

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody detects COX4I1 protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
12% SDS-PAGE
COX4 antibody (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901

GTX628901 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX628901) diluted at 1:1000.

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.
A. 50 μg rat muscle lysate/extract
15% SDS-PAGE
COX4 antibody [GT854] (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhao J et al. Br J Cancer 2021; 124 (2) : 425-436 G3BP1 interacts with YWHAZ to regulate chemoresistance and predict adjuvant chemotherapy benefit in gastric cancer.
Peri? I et al. Neuroscience 2020; 451 : 111-125 Tianeptine Enhances Energy-related Processes in the Hippocampal Non-synaptic Mitochondria in a Rat Model of Depression.
Application : WB
Reactivity : Rat
Dongil P et al. Aging (Albany NY) 2020; 12 (3) : 2275-2301 PAS kinase deficiency reduces aging effects in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations