GeneTex

  • Country / Location Selection

United States (US)

CRM1 antibody [5G3]

Anti-CRM1 antibody [5G3] used in Western Blot (WB). GTX57736
Anti-CRM1 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX57736
Anti-CRM1 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX57736
Anti-CRM1 antibody [5G3] used in Flow cytometry (FACS). GTX57736

Cat. No. GTX57736

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G3

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($499)
See all CRM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cell
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human CRM1 recombinant protein (Position: N966-D1071).

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRM1 , XPO1 , emb , exp1 , exportin 1

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm,Cajal body,Nucleolus

Background

This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-CRM1 antibody [5G3] used in Western Blot (WB). GTX57736

GTX57736 WB Image

WB analysis of various samples using GTX57736 CRM1 antibody [5G3]._x000D_
Lane 1 : rat liver tissue lysate_x000D_
Lane 2 : rat lung tissue lysate_x000D_
Lane 3 : mouse liver tissue lysate_x000D_
Lane 4 : mouse lung tissue lysate_x000D_
Lane 5 : Rabbit IgG_x000D_
Lane 6 : Marker_x000D_
Lane 7 : HepG2 whole cell lysate_x000D_
Lane 8 : SMMC-7721 whole cell lysate_x000D_
Lane 9 : HeLa whole cell lysate_x000D_
Lane 10 :Jukat whole cell lysate
Dilution : 0.5μg/ml
Loading : 50μg per lane

Anti-CRM1 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX57736

GTX57736 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX57736 CRM1 antibody [5G3].
Dilution : 2 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6.0) for 20 mins

Anti-CRM1 antibody [5G3] used in IHC (Paraffin sections) (IHC-P). GTX57736

GTX57736 IHC-P Image

IHC-P analysis of human intestinal cancer tissue using GTX57736 CRM1 antibody [5G3].
Dilution : 2 μg/ml
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6.0) for 20 mins

Anti-CRM1 antibody [5G3] used in Flow cytometry (FACS). GTX57736

GTX57736 FACS Image

FACS analysis of SiHa cells using GTX57736 CRM1 antibody [5G3].
Blue : Primary antibody
Red : Blank
Green : Isotype control
Antibody amount : 1μg/1x10⁶cells

REFERENCE

There are currently no references for CRM1 antibody [5G3] (GTX57736). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CRM1 antibody [5G3] (GTX57736). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 499