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CSRP1 antibody [N1C3]

Anti-CSRP1 antibody [N1C3] used in Western Blot (WB). GTX114344
Anti-CSRP1 antibody [N1C3] used in Immunoprecipitation (IP). GTX114344
Anti-CSRP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114344
Anti-CSRP1 antibody [N1C3] used in Western Blot (WB). GTX114344

Cat. No. GTX114344

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CSRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CSRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619761

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cysteine and glycine rich protein 1 , CRP , CRP1 , CSRP , CYRP , D1S181E , HEL-141 , HEL-S-286

Cellular Localization

Nucleus

Background

This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this gene product occurs in proteins with critical functions in gene regulation, cell growth, and somatic differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CSRP1 antibody [N1C3] used in Western Blot (WB). GTX114344

GTX114344 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
CRP1 antibody
GTX114344 diluted at 1:1000

Anti-CSRP1 antibody [N1C3] used in Immunoprecipitation (IP). GTX114344

GTX114344 IP Image

Immunoprecipitation of CRP1 protein from A549 whole cell extracts using 5 μg of CRP1 antibody [N1C3] (GTX114344).
Western blot analysis was performed using CRP1 antibody [N1C3] (GTX114344) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CSRP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114344

GTX114344 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CSRP1 antibody [N1C3] (GTX114344) at 1:300 dilution.

Anti-CSRP1 antibody [N1C3] used in Western Blot (WB). GTX114344

GTX114344 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
12% SDS PAGE
GTX114344 diluted at 1:1000

REFERENCE

Application Reference

Hayashi K et al. Cell Struct Funct 2023; 48 (1) : 83-98 Role of CRP2-MRTF interaction in functions of myofibroblasts.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169