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CTLA4 antibody

Overexpression
Anti-CTLA4 antibody used in Western Blot (WB). GTX134527
Anti-CTLA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134527
Anti-CTLA4 antibody used in Western Blot (WB). GTX134527

Cat. No. GTX134527

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CTLA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CTLA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909822

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytotoxic T-lymphocyte-associated protein 4 , Cd152 , Ctla-4 , Ly-56

Cellular Localization

Cell membrane

Background

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-CTLA4 antibody used in Western Blot (WB). GTX134527

GTX134527 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody (GTX134527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CTLA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX134527

GTX134527 IHC-P Image

CTLA4 antibody detects CTLA4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
CTLA4 stained by CTLA4 antibody (GTX134527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CTLA4 antibody used in Western Blot (WB). GTX134527

GTX134527 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody (GTX134527) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169