APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)
Product Note
These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
CTRP2 antibody was raised against recombinant human CTRP2.
Purification
Purified by DEAE chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C1q and TNF related 2 , CTRP2 , zacrp2
Cellular Localization
Secreted
Background
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are are expressed in a wide variety of tissues (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation (3).
Database
Research Area
DATA IMAGES
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GTX85071 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX85071 CTRP2 antibody.
Working concentration : 20 μg/ml
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GTX85071 WB Image
WB analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng CTRP2 recombinant protein using GTX85071 CTRP2 antibody.
Working concentration : 1 μg/ml
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GTX85071 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX85071 CTRP2 antibody.
Working concentration : 10 μg/ml
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REFERENCE
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REVIEW
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