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CXCL10 / IP10 antibody [6D4]

Anti-CXCL10 / IP10 antibody [6D4] used in IHC (Frozen sections) (IHC-Fr). GTX54425
Anti-CXCL10 / IP10 antibody [6D4] used in Flow cytometry (FACS). GTX54425

Cat. No. GTX54425

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all CXCL10 / IP10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent

Note :

WB
Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE.

IHC-Fr
Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit.

FACS
For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation.
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IP-10

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C7,CXC motif chemokine ligand 10,CXCL10,IFI10,INP10,IP10,SCYB10,crg2,gIP10,mob1,MIP2,C-X-C motif chemokine ligand 10

Cellular Localization

Secreted

Background

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Anti-CXCL10 / IP10 antibody [6D4] used in IHC (Frozen sections) (IHC-Fr). GTX54425

GTX54425 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue section using GTX54425 CXCL10 / IP10 antibody [6D4].

Anti-CXCL10 / IP10 antibody [6D4] used in Flow cytometry (FACS). GTX54425

GTX54425 FACS Image

FACS analysis of LPS stimulated HUVEC cells using GTX54425 CXCL10 / IP10 antibody [6D4].
Black : Cell only control
Red : Isotype control
Blue : Primary antibody
Antibody amount : 1 μg / 2.5x10⁵ cells

REFERENCE

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REVIEW

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Package List Price ($)
$ 399