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CYB5R3 antibody

Anti-CYB5R3 antibody used in Western Blot (WB). GTX34344
Anti-CYB5R3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34344

Cat. No. GTX34344

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CYB5R3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from human CYB5R3 at 110-190 aa, Internal.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B5R,CYB5R3,DIA1,cytochrome b5 reductase 3

Cellular Localization

Endoplasmic reticulum membrane,Mitochondrion outer membrane,Cytoplasm

Background

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-CYB5R3 antibody used in Western Blot (WB). GTX34344

GTX34344 WB Image

WB analysis of HepG2 cell lysate using GTX34344 CYB5R3 antibody.

Anti-CYB5R3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34344

GTX34344 IHC-P Image

IHC-P analysis of human placenta tissue using GTX34344 CYB5R3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399