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CYP7A1 antibody, C-term

Anti-CYP7A1 antibody, C-term used in Western Blot (WB). GTX81230
Anti-CYP7A1 antibody, C-term used in Western Blot (WB). GTX81230

Cat. No. GTX81230

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
400 μl ($399)
See all CYP7A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CYP7A1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 7 subfamily A member 1 , CP7A , CYP7 , CYPVII

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-CYP7A1 antibody, C-term used in Western Blot (WB). GTX81230

GTX81230 WB Image

WB analysis of HepG2 whole cell lysate using GTX81230 CYP7A1 antibody, C-term.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-CYP7A1 antibody, C-term used in Western Blot (WB). GTX81230

GTX81230 WB Image

WB analysis of K562(lane 1), HepG2(lane 2) cell lysate (35ug/lane) using GTX81230 CYP7A1 antibody, C-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399