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Calbindin antibody

Comparison
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856
Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856

Cat. No. GTX130856

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calbindin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

mouse brain , rat brain

Product Note

Based on sequence homology, this antibody may cross react with CALB2 protein.

Predict Reactivity

Rabbit, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calbindin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calbindin 1 , CALB , D-28K

Background

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856

GTX130856 ICC/IF Image

Calbindin antibody detects Calbindin protein at nucleus and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856

GTX130856 ICC/IF Image

Calbindin antibody detects Calbindin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Calbindin antibody (GTX130856) diluted at 1:1000 and competitor's antibody (sc-7691) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

IMR32 whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Calbindin antibody (GTX130856) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calbindin antibody (GTX130856) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Calbindin protein stained by Calbindin antibody (GTX133218) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169