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Calcineurin B antibody

Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX109127
Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127
Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127
Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX109127
Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127

Cat. No. GTX109127

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Calcineurin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

mouse fetal brain , Rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Calcineurin B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 3 regulatory subunit B, alpha , CALNB1 , CNB , CNB1

Background

Enables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimer's disease and dilated cardiomyopathy. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX109127

GTX109127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calcineurin B antibody (GTX109127) at 1:300 dilution.

Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127

GTX109127 WB Image

Various tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX109127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127

GTX109127 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX109127) diluted at 1:100000.

Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX109127

GTX109127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Calcineurin B(GTX109127) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calcineurin B antibody used in Western Blot (WB). GTX109127

GTX109127 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX109127) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169