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Calcium Sensing Receptor antibody

Anti-Calcium Sensing Receptor antibody used in Western Blot (WB). GTX79178
Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178
Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178
Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178

Cat. No. GTX79178

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Calcium Sensing Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100
ICC/IF 1:10-1:100
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues A(12) L A W H S S A Y G P D Q R A Q(27) of rat CaSR.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11169442

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium-sensing receptor , PCaR1 , RaKCaR

Cellular Localization

Cell membrane

Background

a G protein-coupled receptor that senses extracellular calcium; involved in regulating the secretion of parathyroid hormone, bile and intestinal fluid [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Calcium Sensing Receptor antibody used in Western Blot (WB). GTX79178

GTX79178 WB Image

WB analysis of 25 ug of U251 (lane 1) and rat brain (lane 2) cell lysates using GTX79178 Calcium Sensing Receptor antibody.
Dilution : 1:500

Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178

GTX79178 ICC/IF Image

ICC/IF analysis of U251 cells using GTX79178 Calcium Sensing Receptor antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178

GTX79178 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79178 Calcium Sensing Receptor antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-Calcium Sensing Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79178

GTX79178 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX79178 Calcium Sensing Receptor antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399