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Calmodulin antibody [6D4]

Anti-Calmodulin antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25494
Anti-Calmodulin antibody [6D4] used in Western Blot (WB). GTX25494

Cat. No. GTX25494

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D4

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Chicken, Bacteria
Package
200 μl ($399)
See all Calmodulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF 1:250
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Addition of EGTA to buffers completely inhibits antibody staining.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dictyostelium discoideum

Immunogen

Calmodulin purified from Dictyostelium discoideum.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Research Area

DATA IMAGES

Anti-Calmodulin antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25494

GTX25494 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25494 Calmodulin antibody [6D4]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-Calmodulin antibody [6D4] used in Western Blot (WB). GTX25494

GTX25494 WB Image

WB analysis of purified calmodulin protein using GTX25494 Calmodulin antibody [6D4].

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399