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Calpain 1 antibody [9A4H8D3]

Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in IHC (Paraffin sections) (IHC-P). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in Western Blot (WB). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in IHC (Paraffin sections) (IHC-P). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in Western Blot (WB). GTX23589
Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589

Cat. No. GTX23589

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9A4H8D3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Yeast, Rabbit, Sheep, Bovine, Hamster, Pig, Fish, Arthropod
Package
100 μl ($399)
See all Calpain 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin and it recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified bovine skeletal muscle 80 kDa mu-calpain subunit.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 1 , CANP 1 , CANP1

Cellular Localization

Cytoplasm,Cell membrane

Database

Research Area

DATA IMAGES

Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589

GTX23589 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Calpain 1 antibody [9A4H8D3] used in IHC (Paraffin sections) (IHC-P). GTX23589

GTX23589 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX23589 Calpain 1 antibody [9A4H8D3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calpain 1 antibody [9A4H8D3] used in Western Blot (WB). GTX23589

GTX23589 WB Image

WB analysis of 15 ug of HeLa cell lysate (lane 2), 15 ug of A431 cell lysate (lane 3) using GTX23589 Calpain 1 antibody [9A4H8D3].
Dilution : 1:500

Anti-Calpain 1 antibody [9A4H8D3] used in IHC (Paraffin sections) (IHC-P). GTX23589

GTX23589 IHC-P Image

IHC-P analysis of human kidney tissue using GTX23589 Calpain 1 antibody [9A4H8D3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589

GTX23589 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calpain 1 antibody [9A4H8D3] used in Western Blot (WB). GTX23589

GTX23589 WB Image

WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1), A-431 (Lane 2), MOLT-4 (Lane 3), SK-OV-3 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) using GTX23589 Calpain 1 antibody [9A4H8D3].
Dilution : 2 μg/ml

Anti-Calpain 1 antibody [9A4H8D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23589

GTX23589 ICC/IF Image

ICC/IF analysis of U251 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399