GeneTex

  • Country / Location Selection

United States (US)

Calpain 3 antibody [C1C3]

Anti-Calpain 3 antibody [C1C3] used in Western Blot (WB). GTX113398
Anti-Calpain 3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113398

Cat. No. GTX113398

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Calpain 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036416

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 3 , CANP3 , CANPL3 , LGMD2 , LGMD2A , LGMDD4 , LGMDR1 , nCL-1 , p94

Cellular Localization

Cytoplasm

Background

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 3 antibody [C1C3] used in Western Blot (WB). GTX113398

GTX113398 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX113398 diluted at 1:1000

Anti-Calpain 3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113398

GTX113398 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Calpain 3 (GTX113398) antibody at 1:200 dilution.

REFERENCE

There are currently no references for Calpain 3 antibody [C1C3] (GTX113398). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Calpain 3 antibody [C1C3] (GTX113398). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169